Regulatory
Part:BBa_K817032:Design
Designed by: Yi-Te Lee, Chieh-Mei Wang Group: iGEM12_NTU-Taida (2012-09-19)
PfadBA-TetR(P1040)-pLac
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2154
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3175
Design Notes
We add repressor TetR P1040 and repressor PLac I763027 sequentially in the back of K817002. The double repressor is designed to sharpen the promoter responsive curve.
Source
K817002 – P1040 - I763027