Regulatory

Part:BBa_K817032:Design

Designed by: Yi-Te Lee, Chieh-Mei Wang   Group: iGEM12_NTU-Taida   (2012-09-19)

PfadBA-TetR(P1040)-pLac


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3175


Design Notes

We add repressor TetR P1040 and repressor PLac I763027 sequentially in the back of K817002. The double repressor is designed to sharpen the promoter responsive curve.

Source

K817002 – P1040 - I763027

References